ABSTRACT: The objective of this study was to evaluate the color alteration of four brands of artificial teeth (Art Plus, Trilux, Bionote and Biolux), after immersion in color solutions of coffee, red wine and urucum. We used 80 artificial teeth, which were standardized with 2 mm thickness. Then, they were subjected to finishing and polishing, and divided into 4 groups (n = 05), which were immersed in one of the 3 types of dye, plus distilled water (control). The samples were immersed daily for 4 hours, then they were removed, washed in tap water, dried with absorbent paper and immersed in distilled water for the subsequent hours in a biological glasshouse at 37 ºC, for 21 days. The evaluation of the color change was made in the periods of 0, 7, 14 and 21 days, by means of the tristimulus colorimeter. The data were subjected to the ANOVA and Tukey test with a 5 % significance. The results showed that the urucum was the substance that caused the greatest staining while the coffee and the wine did not show statistical difference. Regarding trademarks, Trilux presented a statistical difference compared to the other commercial brands, revealing colorimetric alteration only in the 14-day period. It was concluded that the composition of the artificial teeth, the type of pigmenting agent and the immersion time determine the color change.

RESUMEN: El objetivo de este estudio fue evaluar la alteración de color de cuatro marcas de dientes artificiales (Art Plus, Trilux, Bionote y Biolux), después de inmersión en soluciones colorantes de café, vino tinto y urucum. Se utilizaron 80 dientes artificiales, los cuales fueron estandarizados con 2 mm de espesor. En seguida, los mismos fueron sometidos al acabado y pulido, y divididos en 4 grupos (n = 5), los cuales fueron inmersos en uno de los 3 tipos de colorante, más agua destilada (control). Las muestras permanecieron inmersas diariamente durante 4 horas, luego fueron retiradas, lavadas en agua corriente, secas con papel absorbente e inmersas en agua destilada por las horas subsiguientes en invernadero biológico a 37 ºC, por 21 días. La evaluación del cambio de color se realizó en los períodos de 0, 7, 14 y 21 días, por medio del colorímetro tristimulo. Los datos fueron sometidos a la prueba ANOVA y Tukey con un 5 % de significancia. Los resultados demostraron que el urucum fue la sustancia que provocó mayor manchado mientras que el café y el vino no presentaron diferencia estadística. En cuanto a las marcas comerciales, el Trilux presentó una diferencia estadística en comparación con las otras marcas comerciales, revelando alteración colorimétrica sólo en el período de 14 días. Se concluyó que la composición de los dientes artificiales, el tipo de agente pigmentante y el tiempo de inmersión determinan el cambio de color.

ABSTRACT The aim of this study was to isolate and identify Candida species from the oral cavity of denture wearers with denture-related stomatitis who were attended at the University Federal of Pará (Belém City, Pará State, Brazil). A total of 36 denture wearers with denture-related stomatitis were included, and type I (50%), type II (33%) and type III (17%) stomatitis were observed. Candida spp. were isolated from 89% of the cases and included five different Candida species. C. albicans was the most frequently recovered species (78% of the cases), followed by C. famata and C. tropicalis. We observed a significant association between Candida species isolation and unsatisfactory denture condition (p = 0.0017). Our results demonstrated the highly frequency of Candida species isolation in denture wearers with denture-related stomatitis and showed the relationship between these species and poor denture maintenance.

OBJETIVO: O objetivo deste estudo foi avaliar a relação entre fatores funcionais e qualitativos no desenvolvimento da estomatite protética (EP) (de acordo com a classificação de Newton) em usuários de dentadura acrílica residentes no norte do Brasil.MATERIAL E MÉTODO: Um total de 99 pacientes, que usavam dentadura superior de resina acrílica parcial ou total, foi incluído neste estudo. Os participantes preencheram um formulário de dados epidemiológicos, que incluiu gênero, idade, fatores locais (hábitos de higiene, retirar a dentadura ao dormir, uso de colutório, condição atual da dentadura, idade da dentadura) e fatores funcionais (dimensão vertical de repouso, dimensão vertical de oclusão, oclusão, retenção, estabilidade estática e dinâmica). Para detectar leveduras, amostras foram coletadas da superfície interna da dentadura e da mucosa palatal em contato com esta. Posteriormente, as amostras foram cultivadas em ágar Sabouraud dextrose, observando-se características macro e microscópicas.RESULTADO: No presente estudo, não foi encontrada relação significante entre gênero e início da doença. Baseada na classificação de Newton, 36,3% dos pacientes apresentaram EP e 89,0% foram colonizados por leveduras; destes indivíduos, 50% tiveram lesões tipo I, 33,3% tiveram lesões tipo II e 16,6% tiveram lesões tipo III. Todos os fatores locais e qualitativos, exceto o uso de colutório, foram clinicamente relevantes para o desenvolvimento da doença.CONCLUSÃO: Estomatite protética em usuários de dentadura do norte do Brasil foi multifatorial, associando fatores locais, funcionais e microbiológicos.

OBJECTIVE: The aim of this study was to evaluate the relationship among functional and qualitative factors in the development of denture stomatitis (DS) (according to Newton's classification) in acrylic-based denture wearers residents from northern Brazil.MATERIAL AND METHOD: A total of 99 patients who wore partial or total acrylic resin-based upper dentures were included in this study. The subjects completed an epidemiological data form that includes the patient's gender, age, local factors (hygiene habits, remove denture to sleep, use of mouthwash, present condition of the denture, age of the denture) and functional factors (vertical dimension at rest, vertical dimension of occlusion, occlusion, retention, and static and dynamic stability). To detect yeasts, samples were collected from the inner surface of the dentures and from the palatal mucosa in contact with it. Subsequently, the samples were cultured on Sabouraud dextrose agar, observing macro and microscopic characteristics.RESULT: In the present study, we did not find any significant relationship between the gender and disease onset. Based on the Newton classification, 36.3% of the patients presented with DS and 89.0% were colonized by yeasts; of these subjects, 50% had type I lesions, 33.3% had type II lesions, and 16.6% had type III lesions. All of the qualitative and local factors, except the use of mouthwash, were clinically relevant to the development of disease.CONCLUSION: Denture stomatitis in denture users in northern Brazil was multifactorial, involving local, functional and microbiological factors.

Introduction The aim of this study was to investigate the effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers. Methods Ten C. albicans isolates recovered from denture wearers were tested using 10% fetal bovine serum with or without 4% R. officinalis essential oil. Results The essential oil from R. officinalis completely inhibited germ tube formation in the investigated C. albicans isolates. Conclusions The results demonstrate that the essential oil of R. officinalis modulates C. albicans pathogenicity through its primary virulence factor (i.e., germ tube formation was suppressed).

RESUMO Quarenta e seis olhos de 23 pacientes diabéticos do Hospital de Clínicas de Franco da Rocha foram examinados e classificados com relação à retinopatia diabética mediante oftalmoscopia indireta e posteriormente angiofluoroscopia para detecção de alterações microvasculares relacionadas à doença, triando o real número de pacientes que necessitam ser submetidos à angiografia fluoresceínica, além de mapear alterações que permitem orientação terapêutica com laser. É um exame de baixo custo e fácil execução, podendo ser amplamente utilizado pelos serviços públicos mais carentes de nosso país.

SUMMARY The authors have studied 23 diabetic patients at the Hospital de Clínicas de Franco da Rocha classifying their retinopathy grade and proceeding angiofluoroscopy to detect the microvascular abnormalities due to the disease. They emphasize the importance of an easy and inexpensiive exam that can help in the diagnosis and orientate the treatment, compatible with the echonomical reality of our country.

RESUMO O estudo de 284 olhos de 142 pacientes diabéticos do HC da cidade de Franco da Rocha insulinodependentes ou não, acompanhados ou não por clínico geral, mostrou nítida prevalência de retinopatia diabética pré-proliferativa e proliferativa nos pacientes insulinodependentes em relação aos não insulinodependentes. A gravidade da retinopatia diabética esteve diretamente relacionada com o tempo de duração do diabete, onde se obteve 48,49% de retinopatia diabética pré-proliferativa e proliferativa nos pacientes diabéticos há mais de 15 anos. Retinopatia diabética proliferativa ocorreu em 14,79% dos pacientes diabéticos. Um questionário empregado aos pacientes demonstrou grande ignorância deles à respeito de sua patologia.

SUMMARY The study of 284 eyes of 142 diabetic patients was conducted in Franco da Rocha’s Clinic Hospital showing cIear prevalence of pre-proliferative and proliferative diabetic retinopathy in the insulin dependent patients in relation to the non insulin dependents ones. The severity of diabetic retinopathy had been directly connected to the duration of the disease, where we could observe 48,49% of pre-proliferative and proliferative diabetic retinopathy in patients with the pathology more than 15 years. Proliferative diabetic retinopathy occurred in 14,79% of diabetic patients. A Survey about the degree of information received by the patients concerning the possibility of becoming blind due to disease showed a lack of knowledge among one third of the patients inquired.